Advanced search
Publication Cover
Biotechnic & Histochemistry Volume 82, 2007 - Issue 1
Submit an article Journal homepage
7,574
Views
515
CrossRef citations to date
0
Altmetric
Original

A two-color acid-free cartilage and bone stain for zebrafish larvae

Dr Mb Walker Stowers Institute for Medical Research, Kansas City, MissouriCorrespondence[email protected]
&
Cb Kimmel University of Oregon, Eugene, Oregon
Pages 23-28 | Received 11 Jul 2006, Published online: 12 Jul 2009
 
Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days

Abstract

Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related Research Data

The mechanism of cartilage subdivision in the reorganization of the zebrafish pectoral fin endoskeleton.
Source: Wiley
Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face
Source: Public Library of Science (PLoS)
Exercise‐induced lordosis in zebrafish Danio rerio (Hamilton, 1822)
Source: Wiley
Skeletal development of the chondrocranium in the tongue sole Cynoglossus semilaevis (Pleuronectiformes: Cynoglossidae).
Source: Wiley
MicroRNA Mirn140 modulates Pdgf signaling during palatogenesis
Source: Springer Science and Business Media LLC
The Roles of RNA Polymerase I and III Subunits Polr1c and Polr1d in Craniofacial Development and in Zebrafish Models of Treacher Collins Syndrome.
Source: Public Library of Science (PLoS)
Transgene-mediated skeletal phenotypic variation in zebrafish
Source: Cold Spring Harbor Laboratory
Thyroid hormone coordinates developmental trajectories but does not underlie developmental truncation in danionins.
Source: Wiley
Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway.
Source: Public Library of Science (PLoS)
Alcian Blue staining of cartilage for electron microscopy. Application of the critical electrolyte concentration principle
Source: Springer Science and Business Media LLC
Histochemical characterization of mucosubstances in bone and soft tissue-tumors.
Source: Wiley
EFTUD2 gene deficiency disrupts osteoblast maturation and inhibits chondrocyte differentiation via activation of the p53 signaling pathway.
Source: BMC
Rmrp Mutation Disrupts Chondrogenesis and Bone Ossification in Zebrafish Model of Cartilage-Hair Hypoplasia via Enhanced Wnt/β-Catenin Signaling
Source: Wiley
Radical change of apoptotic strategy following irradiation during later period of embryogenesis in medaka (Oryzias latipes).
Source: Public Library of Science (PLoS)
Development of Teleost Intermuscular Bones Undergoing Intramembranous Ossification Based on Histological-Transcriptomic-Proteomic Data
Source: Multidisciplinary Digital Publishing Institute
Zebrafish sp7:EGFP: A transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration
Source: Wiley
Inhibition of mmp13a during zebrafish fin regeneration disrupts fin growth, osteoblasts differentiation and Laminin organization
Source: Wiley
Making a master filterer: Ontogeny of specialized filtering plates in silver carp (Hypophthalmichthys molitrix)
Source: Wiley
Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis
Source: The Company of Biologists
Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp.
Source: Springer Science and Business Media LLC
Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning
Source: Elsevier BV
Vertebral fusion in the zebrafish caudal skeleton
Source: Wiley
Craniofacial Development
Source: Wiley
An embryonic staging series up to hatching for Cyprinodon variegatus: An emerging fish model for developmental, evolutionary, and ecological research.
Source: Wiley
Functional analysis of candidate genes in 2q13 deletion syndrome implicates FBLN7 and TMEM87B deficiency in congenital heart defects and FBLN7 in craniofacial malformations
Source: Oxford University Press (OUP)
Endoderm Morphogenesis Reveals Integration of Distinct Processes in the Development and Evolution of Pharyngeal Arches
Source: bioRxiv
Fndc3a (Fibronectin Domain Containing Protein 3A) influences median fin fold development and caudal fin regeneration in zebrafish by ECM alteration
Source: bioRxiv
Knockdown Of Shox in Zebrafish Embryo
Source: Wiley
Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer
Source: bioRxiv
Segmentation pattern of zebrafish caudal fin is affected by developmental temperature and defined by multiple fusions between segments.
Source: Wiley
Screen for Genes in Craniofacial Development
Source: Wiley
Using Zebrafish as a Model System for Studying the Transgenerational Effects of Dioxin
Source: Oxford University Press (OUP)
Signaling Events Downstream of AHR Activation That Contribute to Toxic Responses: The Functional Role of an AHR-Dependent Long Noncoding RNA ( slincR) Using the Zebrafish Model
Source: Environmental Health Perspectives
Screening in larval zebrafish reveals tissue-specific distribution of fifteen fluorescent compounds.
Source: The Company of Biologists
TELEOST MODEL FOR BONE DISEASES
Source: Wiley
A rich diversity of opercle bone shape among teleost fishes
Source: Public Library of Science (PLoS)
Effects of thyroid hormone level alterations on the Weberian apparatus ontogeny of cyprinids (Cyprinidae; Teleostei)
Source: Pleiades Publishing Ltd
Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo
Source: Cold Spring Harbor Laboratory
Evolution and development of facial bone morphology in threespine sticklebacks
Source: Proceedings of the National Academy of Sciences
Evolution of a Unique Predatory Feeding Apparatus: Functional Anatomy, Development and a Genetic Locus for Jaw Laterality in Lake Tanganyika Scale-Eating Cichlids
Source: Springer Nature
Understanding the development of intermuscular bones in teleost: status and future directions for aquaculture
Source: Wiley
Anatomical Assessment of the Adult Skeleton of Zebrafish Reared Under Different Thyroid Hormone Profiles.
Source: Wiley
The mechanical impact of col11a2 loss on joints; col11a2 mutant zebrafish show changes to joint development and function which lead to early onset osteoarthritis
Source: Cold Spring Harbor Laboratory
The complex trophic anatomy of silver carp, Hypophthalmichthys molitrix, highlighting a novel type of epibranchial organ.
Source: Wiley
Alcian blue. Now you see it, now you don't.
Source: Wiley
The MITF paralog tfec is required in neural crest development for fate specification of the iridophore lineage from a multipotent pigment cell progenitor
Source: bioRxiv
Hand/foot splitting and the ‘re-evolution’ of mesopodial skeletal elements during the evolution and radiation of chameleons
Source: Springer Nature
Reduction of skeletal anomalies in meagre ( Argyrosomus regius , Asso, 1801) through early introduction of inert diet
Source: Wiley
Evolution of the Actinopterygian Caudal Fin Skeleton
Source: Wiley
Examination of a palatogenic gene program in zebrafish
Source: Wiley
Comparison the Effect of Ferutinin and 17β-Estradiol on Bone Mineralization of Developing Zebrafish (Danio rerio) Larvae.
Source: MDPI AG
Clearing and staining small vertebrates for demonstrating ossification of the skeletal system using the KOH and glycerine clearing method.
Source: Wiley
Ontogeny of the Spotted Gar Caudal Fin Skeleton
Source: Wiley
Stickleback tooth Patterning
Source: Wiley
Kat2a and Kat2b Acetyltransferase Activity Regulates Craniofacial Cartilage and Bone Differentiation in Zebrafish and Mice.
Source: MDPI AG
Icariin reduces bone loss in a Rankl-induced transgenic medaka (Oryzias latipes) model for osteoporosis.
Source: Wiley
Thyroid hormone in skeletal development
Source: Wiley
Rbm24a and Rbm24b are required for normal somitogenesis.
Source: Public Library of Science (PLoS)
Effects of thyroid hormone level alterations on the development of supraneural series in zebrafish, Danio rerio
Source: Wiley
Using Model Organisms in an Undergraduate Laboratory to Link Genotype, Phenotype, and the Environment.
Source: Informa UK Limited
Anti-osteogenic activity of cadmium in zebrafish
Source: Multidisciplinary Digital Publishing Institute
Inhibition of Notch signaling rescues cardiovascular development in Kabuki Syndrome
Source: Cold Spring Harbor Laboratory
Genetic evidence for prevalence of alloparental care in a socially monogamous biparental cichlid fish, Perissodus microlepis, from Lake Tanganyika supports the “selfish shepherd effect” hypothesis
Source: Wiley
Developmental basis of phenotypic integration in two Lake Malawi cichlids
Source: Springer Science and Business Media LLC
Thyroid hormone modulation during zebrafish development recapitulates evolved diversity in danionin jaw protrusion mechanics
Source: Wiley
Interactions among ryanodine receptor isotypes contribute to muscle fiber type development and function.
Source: The Company of Biologists
Alcian-blue staining and lectin-binding affinites in semithin epon sections
Source: Springer Science and Business Media LLC
Exogenous human beta amyloid peptide interferes osteogenesis through Sox9a in embryonic zebrafish
Source: Springer Science and Business Media LLC
A new Miocene skate from the Central Paratethys (Upper Austria): the first unambiguous skeletal record for the Rajiformes (Chondrichthyes: Batomorphii)
Source: Informa UK Limited

Linking provided by 

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.