Expression of PDGF-A, PDGFRA, integrin subunit alpha V and selectin E is increased in canine cutaneous fibrosarcomas

ABSTRACT

We investigated the expression of platelet derived growth factor alpha (PDGFA); its receptor, PDGFRA; integrin subunit alpha V; and selectin E in cutaneous fibrosarcomas in dogs. Ten cases of canine fibrosarcomas were obtained from the archive of the Department of Pathology, Ondokuz Mayis University, Samsun. Tissue sections were cut and stained with hematoxylin and eosin, Alcian blue-periodic acid Schiff, Masson’s trichrome, and also immunostained. Eight tumors classified as spontaneous fibrosarcomas exhibited interwoven bundles of spindle shaped cells with oval to plump nuclei and scant cytoplasm, while two tumors exhibited features of injection site fibrosarcoma with peripheral infiltration of mononuclear cells and intratumor necrosis. We found that neoplastic cells from all cases exhibited cytoplasmic expression of PDGFA, and cytoplasmic and nuclear staining for PDGFRA. Integrin subunit alpha V immunostaining was observed in all cases, while selectin E expression was observed in vascular endothelial cells and neoplastic cells. A significant positive correlation was found between the expression of PDGFA and integrin subunit alpha V. Our findings indicate that PDGFA, PDGFRA, integrin subunit alpha V and selectin E are expressed strongly in canine cutaneous fibrosarcomas and may contribute to tumor progression.

KEYWORDS:

• Canine
• fibrosarcoma
• integrin subunit alpha V
• platelet derived growth factor alpha
• selectin E

Acknowledgments

Ishtiaq Ahmed thanks the Turkish Scientific and Technological Research Council (Tubitak), Turkey for providing a scholarship for doctoral study and the University of Veterinary & Animal Sciences, Lahore, Pakistan for granting study leave. The manuscript is a part of the doctoral research conducted at the Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey.

Disclosure statement

The authors declare no conflict of interest.