Cytotoxic isoquinoline quinones from sponges of the genus Petrosia

Abstract

Bioassay-guided fractionation of two Philippine sponges of the genus Petrosia has resulted in the isolation of the novel natural product cribrostatin 7 (1) and the known compounds renierone (2) and O-demethylrenierone (3). The structures of these isoquinoline quinones were determined by interpretation of spectroscopic data. Compounds 1, 2 and 3 were cytotoxic against the HCT 116 human colon carcinoma cell line with IC₅₀ values of 45, 24 and 34 µg/mL, respectively.

Keywords:

• Marine sponge
• Petrosia sp.
• Isoquinoline quinone
• Cribrostatin 7
• Cytotoxic activity

Acknowledgment

The authors gratefully acknowledge support provided by NIH grant CA36622 (C.M.I.). Funding for the Varian Mercury 400 MHz NMR spectrometer was provided through NIH Grant RR14768. FAB mass spectrometry was performed by Dr. Elliot M. Rachlin on a Finnegan Mat 95 funded by NSF grant CHE-9002690 and the University of Utah Institutional Funds Committee. The authors would also like to thank Dr. J.N.A. Hooper (Queensland Museum) for assistance with sponge taxonomy and Dr. D.A. Jones (Huntsman Cancer Institute) for the use of cell lab facilities.