ABSTRACT
We have synthesized highly exfoliated graphene oxide (GO) and used it for the fabrication of electrochemical immunosensor. The thin films of GO were spin coated onto ITO/glass substrate and thereafter Bovine Serum Albumin (BSA) antibodies (Ab) were immobilized using EDC-NHS binding chemistry to design immunobioelectrode. The graphene oxide films were characterized using different spectroscopic techniques viz FT-IR, UV-Vis spectroscopy and HRTEM. The electrochemical activity of designed immunoelectrodes was studied by cyclic voltammetry (CV) that revealed a quasi reversible behaviour of graphene oxide films. The differential pulse voltammetry (DPV) measurements of the fabricated immunoelectrodes showed successful detection of analyte at very low concentration ranges (50 fg/mL–100 ng/mL). The present study entails the potential of GO for the development of various immunosensors for different diseases.
Acknowledgments
We gratefully acknowledge the DNPL for his constant encouragement and providing us with research facilities. We thank Ms. Meenakshi Choudhary (Ph.D. scholar, JNU) for her guidance and support.
Funding
The work is funded by NANO-SHE project BSC-0112 and DST Inspire Faculty Award (IFA12-CH-31).