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Abstract
We tethered an anthracene group to one end of oligonucleotide to make anthracene–ODN conjugates (5′AntODN15 and 3′AntODN25). The sequences of the conjugates were designed to hybridise to adjacent sites of the template with their anthracene units stacked each other. The conjugates dimerised only in the presence of the template by light irradiation. The dimerisation efficiency was affected by one-base mismatch in the tandem duplexes. Furthermore, we studied the signal amplification experiments under thermal cycling in the presence of the targets with full match and mismatch sequences.
Acknowledgements
This work was partially supported by a grant-in-aid for JSPS fellow (18809000) (M.M) and for Scientific Research (B) (20350038) from JSPS (T.I.). The authors are also grateful to the support from PRESTO, JST (T.I.).