Induction of Partial Protection in Mice after Oral Administration of Lactococcus lactis Producing Brucella abortus L7/L12 Antigen

Daniela S. Pontes Laboratório de Genética Celular e Molecular, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, CP 486, CEP, MG, 30161-970, Belo Horizonte, Brazil

Fernanda A. Dorella Laboratório de Genética Celular e Molecular, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, CP 486, CEP, MG, 30161-970, Belo Horizonte, Brazil

Luciana A. Ribeiro Unité de Recherches Laitières et de Génétique Appliquée (URLGA-INRA), Domaine de Vilvert, cedex, 78352, Jouy-en-Josas, France

Anderson Miyoshi Laboratório de Genética Celular e Molecular, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, CP 486, CEP, MG, 30161-970, Belo Horizonte, Brazil

Yves Le Loir Laboratoire de Microbiologie (ENSA INRA UMR), 65, rue de Saint Brieuc CS, cedex, 35042, Rennes, France

Alexandra Gruss Unité de Recherches Laitières et de Génétique Appliquée (URLGA-INRA), Domaine de Vilvert, cedex, 78352, Jouy-en-Josas, France

Sérgio C. Oliveira Laboratório de Imunologia de Doenças Infecciosas, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, CP 486, CEP, MG, 30161-970, Belo Horizonte, Brazil

Philippe Langella Unité de Recherches Laitières et de Génétique Appliquée (URLGA-INRA), Domaine de Vilvert, cedex, 78352, Jouy-en-Josas, France

Vasco Azevedo Laboratório de Genética Celular e Molecular, Department of General Biology, Institute of Biological Sciences, Federal University of Minas Gerais, CP 486, CEP, MG, 30161-970, Belo Horizonte, BrazilCorrespondence[email protected]

show all

Abstract

The Brucella abortus ribosomal protein L7/L12 is an immunodominant antigen and an interesting candidate for the development of oral live vaccines against brucellosis. Here, a recombinant Lactococcus lactis strain producing L7/L12 under the control of nisin inducible promoter was orally administered to BALB/c mice. Significant levels of anti-L7/L12 specific IgA detected in feces revealed an induced local humoral immune response. However, serum analysis did not reveal any anti-L7/L12 antibodies suggesting the absence of a systemic response. Nevertheless, the vaccinated mice showed a partial protective immunity against B. abortus virulent strain (S2308) challenged by intraperitoneal inoculation.

Keywords::

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Induction of Partial Protection in Mice after Oral Administration of Lactococcus lactis Producing Brucella abortus L7/L12 Antigen

Information for

Open access

Opportunities

Help and information

Induction of Partial Protection in Mice after Oral Administration of Lactococcus lactis Producing Brucella abortus L7/L12 Antigen

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature