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Abstract
Biotinylated immunoliposomes were prepared by a non-covalent (biotin-streptavidin) coupling procedure and conjugated to the OX26 monoclonal antibody directed against the rat transferrin receptor. In vitro, these biotinylated immunoliposomes were used to by-pass P-glycoprotein in multidrug-resistant RBE4 brain capillary endothelial cells and thereby to achieve 2- to 3-fold higher intracellular accumulation of liposomal daunomycin as compared to free drug. The extent of cellular uptake of liposomal daunomycin was dose- and time-dependent, was inhibited by competition with unbound OX26 and was associated with a pharmacological (i.e. cytotoxic) effect. Cytotoxic effects of liposomal formulations of daunomycin, in contrast to the free drug, were apparent only after prolonged incubation periods being indicative of a slow intracellular unpacking and release of liposomal daunomycin. Pharmacokinetics and tissue distribution studies in the rat revealed brain accumulation of daunomycin in OX26-immunoliposomes to higher levels as compared to brain uptake of free daunomycin, or daunomycin incorporated within pegylated liposomes or within unspecific IgG2a isotype control immunoliposomes. Such OX26-mediated effects were not observed in other tissues such as spleen, liver, muscle or kidney.
| Abbreviations | ||
| PEG | = | poly(ethylene glycol) |
| PEG-DSPE | = | PEG derivatized distearoylphosphatidylethanolamine |
| bio-PEG-DSPE | = | biotinylated PEG-DSPE |
| DSPC | = | distearoylphosphatidylcholine |
| OX26 mAb | = | OX26 monoclonal antibody to the rat transferrin receptor |
| OX26-SA | = | OX26 mAb conjugated to streptavidin |
| SRB | = | sulforhodamine B |
| Abbreviations | ||
| PEG | = | poly(ethylene glycol) |
| PEG-DSPE | = | PEG derivatized distearoylphosphatidylethanolamine |
| bio-PEG-DSPE | = | biotinylated PEG-DSPE |
| DSPC | = | distearoylphosphatidylcholine |
| OX26 mAb | = | OX26 monoclonal antibody to the rat transferrin receptor |
| OX26-SA | = | OX26 mAb conjugated to streptavidin |
| SRB | = | sulforhodamine B |