Paclitaxel-loaded PLGA nanoparticles surface modified with transferrin and Pluronic^®P85, an in vitro cell line and in vivo biodistribution studies on rat model

Abstract

The development of multidrug resistance (due to drug efflux by P-glycoproteins) is a major drawback with the use of paclitaxel (PTX) in the treatment of cancer. The rationale behind this study is to prepare PTX nanoparticles (NPs) for the reversal of multidrug resistance based on the fact that PTX loaded into NPs is not recognized by P-glycoproteins and hence is not effluxed out of the cell. Also, the intracellular penetration of the NPs could be enhanced by anchoring transferrin (Tf) on the PTX-PLGA-NPs. PTX-loaded PLGA NPs (PTX-PLGA-NPs), Pluronic^®P85-coated PLGA NPs (P85-PTX-PLGA-NPs), and Tf-anchored PLGA NPs (Tf-PTX-PLGA-NPs) were prepared and evaluted for cytotoxicity and intracellular uptake using C6 rat glioma cell line. A significant increase in cytotoxicity was observed in the order of Tf-PTX-PLGA-NPs > P85-PTX-PLGA-NPs > PTX-PLGA-NPs in comparison to drug solution. In vivo biodistribution on male Sprague–Dawley rats bearing C6 glioma (subcutaneous) showed higher tumor PTX concentrations in animals administered with PTX-NPs compared to drug solution.

Keywords::

• Paclitaxel
• transferrin-anchored PLGA NPs
• C6 glioma
• Pluronic^®P85
• multidrug resistance

Acknowledgments

This research was supported by Indian Council of Medical Research (ICMR) (45/33/2006-Pha/BMS), India. The authors are also thank Intas Biopharma, India for providing the cell culture facilities and Sun Pharma Advanced Research Company Ltd. (SPARC) for providing free gift sample of PTX.

Declaration of interest: The authors report no conflicts of interest.