Polymeric nanoparticulate system augmented the anticancer therapeutic efficacy of gemcitabine

Abstract

Gemcitabine hydrochloride is an anticancer nucleoside analogue indicated in clinic for the treatment of various solid tumors. Although this drug has been demonstrated to display anticancer activity against a wide variety of tumors, it is needed to be administered at high doses to elicit the required therapeutic response, simultaneously leading to severe adverse effects. We hypothesized that the efficient delivery of gemcitabine to tumors using a biodegradable carrier system could reduce the dose required to elicit sufficient therapeutic response. Thus, we have developed a nanoparticle formulation of gemcitabine suitable for parenteral administration based on the biodegradable polymer poly(octylcyanoacrylate) (POCA). The nanoparticles were synthesized by anionic polymerization of the corresponding monomer. Two drug loading methods were analyzed: the first one based on gemcitabine surface adsorption onto the preformed nanoparticles, and the second method being gemcitabine addition before the polymerization process leading to drug entrapment in the polymeric network. A detailed investigation of the capabilities of the polymer particles to load this drug is described. Gemcitabine entrapment into the polymer matrix yielded a higher drug loading and a slower drug release profile as compared with drug adsorption procedure. The main factors determining the gemcitabine incorporation to the polymer network were the nanoparticles preparation procedure, the monomer concentration, the surfactant concentration, the pH, and the drug concentration. The release kinetic of gemcitabine was found to be controlled by the pH and the type of drug incorporation. The cytotoxicity studies performed on L1210 tumor cells revealed a similar anticancer activity of the gemcitabine-loaded POCA (GPOCA) nanoparticle as free gemcitabine. Following intravenous administration into the mice bearing L1210 wt subcutaneous tumor, the GPOCA nanoparticles displayed significantly greater anticancer activity compared to free gemcitabine; this has been additionally confirmed by histology and immunohistochemistry studies, suggesting the potential of GPOCA for the efficient treatment of cancer.

Keywords::

• Antitumor activity
• biodegradable polymers
• colloidal drug delivery systems
• controlled release
• cytotoxicity
• immunohistochemistry
• poly(alkylcyanoacrylates)

Acknowledgments

Henkel Loctite (Ireland) is gratefully acknowledged for the kind supply of octylcyanoacrylate monomer. L1210 cell line is a gift from Prof. Charles Dumontet, University of Lyon, France. The financial support of the “Agence Nationale de la Recherche” (ANR, grant SYLIANU) and of the CNRS (grant “Ingénieur de valorisation”) is also acknowledged, as is the post-doctoral fellowship to LH Reddy, and the associate CNRS researcher fellowship to JL Arias from the Univ. Paris-Sud (France). The authors would like to thank Dr. Jack-Michel Renoir and Dr. Veronique Marsaud for their help in carrying out the histology and immunohistochemistry studies.

Declaration of interest: The authors report no conflicts of interest.