Plasma Hemopexin Activity in Pregnancy and Preeclampsia

Winston W. Bakker Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The NetherlandsCorrespondence[email protected]

Rogier B. Donker Division of Medical Biology, Department of Pathology of Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands; Departments of Obstetrics and Gynecology, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Albertus Timmer Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Mariëlle G. van Pampus Departments of Obstetrics and Gynecology, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Willem J. van Son Department of Internal Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Jan G. Aarnoudse Departments of Obstetrics and Gynecology, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Harry van Goor Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Klary E. Niezen-Koning Department of Pediatrics, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Gerjan Navis Department of Internal Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Theo Borghuis Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Rianne M. Jongman Departments of Pathology and Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

Marijke M. Faas Division of Medical Biology, Department of Pathology of Laboratory Medicine, University Medical Center of Groningen and University of Groningen, Groningen, The Netherlands

show all

Abstract

Objective: Plasma hemopexin activity, associated with increased vascular permeability, was evaluated in healthy pregnant and non-pregnant women and in pre-eclamptic women. Methods: Hemopexin activity and the hemopexin inhibitor, extracellular ATP, were assayed in plasma from pregnant (n = 10), preeclamptic (n = 9), and non-pregnant women (n = 10) using standard methods. Abdominal fascia tissue fragments from preeclamptic and pregnant women were immunohistochemically stained for vascular ecto-apyrase or ecto-5′nucleotidase. Results: The data show significantly enhanced Hx activity exclusively in plasma from pregnant women and significantly enhanced plasma ATP in pre-eclamptic women compared with the other groups. Dephosphorylation of preeclamptic plasma resulted in reactivation of Hx activity. Fascia tissue-samples from preeclamptic women showed reduced ecto-apyrase activity and enhanced ecto-5′nucleotidase activity compared to pregnant women. Conclusion: Enhanced hemopexin activity may be associated with normal pregnancy, but not with preeclampsia. Decreased hemopexin in pre-eclamptic patients may be due to enhanced plasma ATP, which is possibly promoted by diminished activity of vascular ecto-apyrase.

Keywords:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Plasma Hemopexin Activity in Pregnancy and Preeclampsia

Information for

Open access

Opportunities

Help and information

Plasma Hemopexin Activity in Pregnancy and Preeclampsia

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature