Surface modification of Poly Hydroxybutyrate (PHB) nanofibrous mat by collagen protein and its cellular study

Abstract

In this study, the effects of CO₂ plasma surface modification and cross linking with collagen on the properties of poly-[3-hydroxybutyrate] (PHB) nanofibres were investigated. The samples were evaluated by attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR), scanning electron microscope (SEM), contact angle and finally cell culture. ATR–FTIR structural and SEM analyses showed the presence of functional groups and coating on the nanofibrous surfaces. The 85° difference was obtained in the contact angle analysis, obtained for the collagen-cross-linked nanofibrous mat than the un-modified nanofibrous mat. Cellular investigation showed better adhesion of collagen-cross-linked nanofibrous samples than other samples. The collagen-cross-linked mats are a suitable scaffold for enhancing the cell adhesion in nerve regeneration.

Keywords:

• Nanofibrous Poly hydroxybutyrate (PHB)
• Surface modification
• Plasma treatment
• Collagen
• Cross linking
• Cellular investigation