Greener synthesis of cerium oxide nanoemulsion using pollen grains of Brassica napus and evaluation of its antitumour and cytotoxicity properties.

ABSTRACT

In this project, the CeO₂NP_s were synthesized using pollen grains of Brassica napus as a capping agent and reducing agent. The nanoceria were characterized by FTIR, FESEM, TEM, PXRD, and DLS. To increase the bioavailability, the nanoemulsion of cerium nanoparticle was prepared. The TEM images have revealed particles with spherical morphology and mean particle sizes of ca. 23.2±4.0 nm. The prepared sample after calcination at 500 °C showed the characteristic peaks of nanoceria with a good crystallinity. Chorioallantoic membrane (CAM) test results showed by increasing the concentration of biosynthesized CeO₂NP_s, the size and number of blood vessels were reduced. Also, the cytotoxicity test using MTT showed little toxicity of the biosynthetic CeO₂NP_s on the human foreskin fibroblast cell, whereas synthetic CeO₂NP_s destroyed ovarian cancer cells (A2780) which depends on the time and concentration of the NPs. The results of this research show excessive promise for drug treatments and pharmacological technology.

KEYWORDS:

• Cerium oxide nanoparticles
• nanoemulsion
• Brassica napus
• cell toxicity
• antitumor

Acknowledgments

The authors appreciatively acknowledge for support and assistance provided by Islamic Azad University.

Disclosure statement

The authors declare that there are no conflicts of interest.