Incorporation of Boswellia sacra essential oil into chitosan/TPP nanoparticles towards improved therapeutic efficiency

ABSTRACT

The aim of this survey was to fabrication of chitosan nanoparticle was loaded by Boswellia sacra essential oil (BS-CNPs) and investigation of its pharmacological activities.BS-CNPs was synthesized by ionic gelation method and characterized. BS-CNPs-mediated cell viability and cell death were studied by MTT, qPCR, AO/PI staining and flow cytometery. Anti-angiogenesis properties were evaluated by CAM and scratch assay. Anti-oxidant properties were assessed by ABTS, DPPH, FRAP and ROS assay. The BS-CNPs (80.13 nm, ζ-p 39.20 mv and %EE: 82.7%) exhibited selective cytotoxicity on HepG2 cells (IC₅₀:62.94 μg/ml). BS-CNPs induced the apoptosis by up-regulation of P53, Cas-3 and 9. Increasing the rate of free radical scavenging and intracellular ROS confirmed the anti and pro-oxidant potential of BS-CNPs outside and inside of cancer cells.Inhibition (92.3%) of cell migration, reduction of angiogenic factors showed anti-angiogenic effects of BS-CNPs.BS-CNPs due tounique anti-cancer properties are suitable for therapeutic targets in cancer studies.

KEYWORDS:

• Angiogenesis
• apoptosis
• boswellia sacra essential oil
• chitosan nanoparticle
• cytotoxic

Acknowledgments

The authors very much appreciate the support provided by the Islamic Azad University, Mashhad, Iran, in the conducting of the present research.

Disclosure statement

The authors have declared no conflict of interest.