Peroxynitrite promotes mitochondrial permeability transition-dependent rapid U937 cell necrosis: Survivors proliferate with kinetics superimposable on those of untreated cells

Abstract

A short term exposure to peroxynitrite promotes a time- and concentration-dependent lethal response in U937 cells. The mode of cell death was necrosis and rapid (within minutes) cell lysis was found to occur via a mechanism involving mitochondrial permeability transition. Apoptosis was not detected in cells exposed to low levels of peroxynitrite, or in cells which survived a treatment with toxic amounts of peroxynitrite, neither after the 60 min exposure nor following increasing time intervals of growth in fresh culture medium. Rather, cells treated with peroxynitrite concentrations which were not immediately lethal, as well as the survivors of treatments with toxic levels of peroxynitrite, proliferated with kinetics superimposable on those observed in untreated cells.

Keywords:

• Peroxynitrite
• U937 cells
• cell death
• necrosis
• mitochondrial permeability transition