Involvement of protein kinase Cδ in the activation of NADPH oxidase and the phagocytosis of neutrophils

Abstract

This experiment was performed to clarify the role of protein kinase C (PKC) δ in NADPH oxidase-dependent production and actin polymerization followed by phagocytosis in neutrophils. Bovine neutrophils and human neutrophil-like differentiated HL-60 (dHL-60) cells were stimulated with serum-opsonized zymosan (OZ) and fMet–Leu–Phe (fMLP), respectively. Rottlerin, a specific inhibitor of PKCδ, attenuated the production of from NADPH oxidase in both neutrophils and dHL-60 cells. However, it did not inhibit the translocation of p47^phox from the cytosol to the membrane in either type of cell or the phosphorylation of p47^phox in dHL-60 cells. GF109203X (GFX), an inhibitor of cPKC, attenuated not only the production of but also the translocation of p47^phox in both cells. Furthermore, rottlerin significantly attenuated the ingestion of opsonized particles and the formation of F-actin in OZ-stimulated neutrophils, whereas, GFX did not affect those phagocytic processes. These results suggest that both PKCδ and cPKC regulate NADPH oxidase through different pathways, but only PKCδ regulates the phagocytic function in neutrophils.

• cPKC
• NADPH oxidase
• neutrophil
• phagocytosis
• PKCδ