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Abstract
The present study is designed to investigate the effect of pre-conditioning with 35% O2 on PC12 cell death induced by hypoxia. This study investigated whether 35% O2 pre-conditioning for 3 h, followed by 12 h recovery, can protect PC12 cells against death induced by subsequent exposure to hypoxia for 72 h. The result showed that pre-conditioning with 35% O2 partly blocked the decrease in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction induced by hypoxia in PC12 cells. PC12 cells pre-conditioned with 35% O2 could generate a small quantity of reactive oxygen species (ROS), which activated the extracellular signal-regulated kinase (ERK) signalling pathway, then the over-expression of the B-cell lymphoma/leukaemia-2 (Bcl-2) was induced, which subsequently protected PC12 cell against death resulting from hypoxia exposure. In conclusion, 35% O2 pre-conditioning could protect PC12 cells against hypoxic insult.
| Abbreviations | ||
| DMEM | = | Dulbecco's modified Eagle's medium |
| SiRNA | = | small interfering RNA |
| PYR | = | pyrogallol |
| CAT | = | catalase |
| H2O2 | = | hydrogen peroxide |
| TEM | = | 4-hydroxyl-tempol |
| PD | = | PD98059 |
| DCFH-DA | = | dichlorofluorescein diacetate |
| Het | = | dihydroethidium |
| H7 | = | 1-5-isoquinolinesulphonyl-2-methypoperazine |
| DMSO | = | dimethyl sulphoxide |
| WO | = | wortmannin |
| MTT | = | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide |
| DCF | = | dichlorofluorescein |
| ROS | = | reactive oxygen species |
| IPC | = | ischemic pre-conditioning |
| PC12 | = | pheochromocytoma |
| PMSF | = | phenylmethylsulphonylfluoride |
| ERK | = | extracellular signal-regulated kinase |
| MAPK | = | mitogen-activated protein kinase |
| PKC | = | protein kinase C |
| PKA | = | protein kinase A |
| PI3K | = | phosphoinositide-3-kinase |
| EGTA | = | ethylene glycol tetraacetic acid |
| CREB | = | cyclic AMP-responsive element binding protein |
| Bcl-2 | = | B-cell lymphoma/leukaemia-2 |
| NF-B | = | nuclear factor B |
| DEPC | = | diethyl-pyrocarbonate |
| AMV-RT | = | avian myeloblastosis virus reverse transcriptase. |
| Abbreviations | ||
| DMEM | = | Dulbecco's modified Eagle's medium |
| SiRNA | = | small interfering RNA |
| PYR | = | pyrogallol |
| CAT | = | catalase |
| H2O2 | = | hydrogen peroxide |
| TEM | = | 4-hydroxyl-tempol |
| PD | = | PD98059 |
| DCFH-DA | = | dichlorofluorescein diacetate |
| Het | = | dihydroethidium |
| H7 | = | 1-5-isoquinolinesulphonyl-2-methypoperazine |
| DMSO | = | dimethyl sulphoxide |
| WO | = | wortmannin |
| MTT | = | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylterazolium bromide |
| DCF | = | dichlorofluorescein |
| ROS | = | reactive oxygen species |
| IPC | = | ischemic pre-conditioning |
| PC12 | = | pheochromocytoma |
| PMSF | = | phenylmethylsulphonylfluoride |
| ERK | = | extracellular signal-regulated kinase |
| MAPK | = | mitogen-activated protein kinase |
| PKC | = | protein kinase C |
| PKA | = | protein kinase A |
| PI3K | = | phosphoinositide-3-kinase |
| EGTA | = | ethylene glycol tetraacetic acid |
| CREB | = | cyclic AMP-responsive element binding protein |
| Bcl-2 | = | B-cell lymphoma/leukaemia-2 |
| NF-B | = | nuclear factor B |
| DEPC | = | diethyl-pyrocarbonate |
| AMV-RT | = | avian myeloblastosis virus reverse transcriptase. |