Targeting of Liposomes to Human Keratinocytes Through Adhesive Peptides from Immunoglobulin Domains in the Presence of IFNM-γ

Abstract

T-cell adhesion is often dictated by the presence of intercellular adhesion molecule-1 (ICAM-1) on the target cell surface. Reconstitution of P 0 protein into liposomes increases adhesion to melanoma cells expressing ICAM-1. In our study, the effect of peptides derived from P 0 protein and leukocyte function associated-antigen 1 (LFA-1) on IFN-γ-stimulated human keratinocytes was investigated. Covalently linked P 0 -peptide-1, from the Ig-like domain, increased specific liposome binding to IFN-γ-stimulated keratinocytes in a dose-dependent manner. C-terminal-derived P 0 -peptide-3 increased liposome binding nonspecifically. LFA-1 and RGD peptides had no apparent effect. P 0 -peptide-1 is thus a potential targeting ligand for liposomal drug delivery to ICAM-1 expressing keratinocytes in inflammatory dermatoses.

Keywords:

• Icam-1
• Keratinocytes
• Liposomes
• Targeted Delivery