Preparation and In Vitro Evaluation of bFGF-Loaded Chitosan Nanoparticles

Meltem Cetin Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey

Yesim Aktas Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey

Imran Vural Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey

Yilmaz Capan Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkey

Lale A. Dogan Department of Basic Oncology, Faculty of Medicine, Institute of Oncology, Hacettepe University, Ankara, Turkey

Memed Duman Department of Chemical Engineering and Bioengineering Division, Hacettepe University, Ankara, Turkey

Turgay Dalkara Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey

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Abstract

The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions.

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Preparation and In Vitro Evaluation of bFGF-Loaded Chitosan Nanoparticles

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Preparation and In Vitro Evaluation of bFGF-Loaded Chitosan Nanoparticles

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