DBBF Modified Human Placenta Hemoglobin through the α Chains

Abstract

Hemoglobin-based blood substitute is widely studied. As the starting materials, hemoglobin (Hb) is mainly supplied from outdated human blood and animal sources. But there are many disadvantages. As the alternative source, human placenta hemoglobin (PHb) has its own advantages. We take the lead in being occupied in the research of blood substitutes based on the placenta blood. Purified PHb readily undergoes dissociation to form two dimers that are easily filtered by the kidneys and have high 0₂ affinity (low P₅₀), failing to transport 0₂ to tissues. As a first step, to enhance PHb stability and P₅₀, we first adopted DBBF to modify deoxyPHb leading to DBBF-αPHb. According to detection, we knew that the PHb stability was improved, 0₂ affinity of the PHb solution was reduced, DBBF-αPHb had the appropriate capacity of oxygen-carrying and oxygen-unloading, and DBBF-αPHb kept the biological activity well. On the whole DBBF-αPHb could be considered as a first step toward the synthesis of a potential blood substitute.

Keywords:

• DBBF
• hemoglobin
• blood substitute
• human placenta hemoglobin