ON-CHIP ELECTROCHEMICAL DETERMINATION OF CEFEPIME

Abstract

Cefepime is an important fourth-generation cephalosporin, having a broad antimicrobial spectrum. This article describes a new application of microfabricated chip with integrated Pt microelectrodes, for the electrochemical detection (ECD) of cefepime. In this analysis, electro-oxidation of cefepime was investigated on an unmodified Pt microelectrode in acetate buffer at pH 4.5. Differential pulse voltammetry (DPV) along with cyclic voltammetry (CV) was performed as a major read out technique. With this method, a linear calibration curve for 25–150 μM with a limit of detection of 15 μM was obtained. This investigation presents a simple methodology and rapid results that, within the above calibration range, can be obtained without any pretreatment of the analyte.

Keywords:

• cefepime
• differential pulse voltammetry
• electro-oxidation
• on-chip detection