2
Views
1
CrossRef citations to date
0
Altmetric
Research Article

Relative Contributions of α4 and αL Integrins to IL-4-Induced Leukocyte Rolling and Adhesion

, &
Pages 655-668 | Published online: 10 Jul 2009
 

Abstract

Objective: To delineate the relative contributions of α4 and α L to mediate interleukin-4 (IL-4) induced leukocyte rolling, and the subsets of leukocytes that use these pathways to adhere.

Methods: Intravital microscopy was used to examine leukocytes in venules of cremaster muscles of mice receiving intrascrotal injections of IL-4. α4 and αL monoclonal antibodies (mAbs) were administrated either prior to (prophylactic) or 24 h following (therapeutic) treatment with IL-4. In addition, fluorescent microspheres coated with mAbs directed against CD4, CD8, or Gr-1 were injected into mice and the number of subset-specific adherent leukocytes was measured.

Results: Prophylactic inhibition of α4 and αL integrins prevented IL-4-induced leukocyte rolling flux (p <. 05) and increased leukocyte rolling velocity twofold (p <. 05), respectively, while blocking either integrin eliminated IL-4-induced leukocyte adhesion (p <. 05). In contrast, therapeutic administration of both anti-α4 and anti-αL mAbs was necessary to completely inhibit IL-4-induced leukocyte adhesion (p <. 05). Furthermore, CD8+ and Gr-1+ leukocytes utilized α4 and αL to adhere to postcapillary venules, whereas CD4+ leukocytes primarily utilized α4.

Conclusions: Following tissue activation with IL-4, α4 and αL initiate the attachment and deceleration, respectively, of leukocytes during rolling, and are responsible for mediating the adhesion CD4+, CD8+, Gr-1+ leukocytes.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.