1
Views
0
CrossRef citations to date
0
Altmetric
Articles

Tissue Bioenergetics and Microvascular Perfusion Are Impaired in Rat Ileal Mucosa in Normotensive Sepsis

&
Pages 49-56 | Received 24 Jan 2005, Accepted 29 Aug 2005, Published online: 10 Jul 2009
 

Abstract

Objective: Sepsis is a systemic inflammatory response to a bacterial infection. Inflammation may result in injury to the small bowel and an increase in translocation of bacteria and toxins across the mucosal barrier, which may contribute to the progression of sepsis. Microcirculatory perfusion or cytopathic hypoxia may cause impairment of tissue bioenergetics and injury in sepsis. The objective of this study was to determine if sepsis is associated with microcirculatory hypoperfusion and impaired tissue bioenergetics in the ileal mucosa.

Materials and Methods: Sprague-Dawley rats were randomized to cecal ligation and perforation (sepsis group, n = 12) or control group (n = 14) and received arterial and venous catheters and fluid resuscitation. Following 24 h, rats were anesthetized with isoflurane and the ileum was prepared for intravital microscopy. Images of NADH fluorescence, which is an index of tissue bioenergetics, central arterial diameter, red cell velocity, red cell flux, and average intercapillary area, were recorded in 6–9 villi in each rat.

Results: Central arterial red cell flux (control 277 ± 30 cell/s, sepsis 108 ± 13 cells/s, p < .05), diameter (control 10.4 ± 0.4 μ m, sepsis 8.2 ± 0.3 μ m, p < .05) and red cell velocity (control 590 ± 47 μ m/s, sepsis 449 ± 63 μ m/s, p < .05) were decreased while average intercapillary area (control 815 ± 171 μ m2, sepsis 1412 ± 364 μ m2, p < .05) and NADH fluorescence (control 116 ± 6 AIU, sepsis 154 ± 9 AIU, p < .05) were increased at the villus tip in the sepsis group.

Conclusion: Sepsis is associated with bioenergetic impairment and capillary hypoperfusion at the villus tip and a decrease in red cell flux in the central arteriole.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.