Two-Dimensional Solid-State NMR Applied to a Chimeric Potassium Channel

Abstract

Solid-state NMR (ssNMR) represents a spectroscopic method to study membrane protein structure and dynamics in lipid bilayers. We present two-dimensional correlation experiments conducted on a fully [¹³C,¹⁵N] labeled version of a chimeric potassium (KcsA-Kv1.3) channel. Data obtained by using two different ion concentrations suggest a structural conservation of the selectivity filter region. SsNMR experiments conducted at two different temperatures point to differential molecular dynamics of the channel.

Key Words: :

• Ion channel
• Membrane protein
• Solid-state NMR
• Toxin.