Adiponectin identified as an agonist for PAQR3/RKTG using a yeast-based assay system

Abstract

The PAQR family of proteins comprises an intriguing group of newly discovered receptors. Although the agonist is known for 5 of the 11 human PAQRs, most are considered “orphan” receptors. We developed a yeast-based assay system for PAQR receptor activity that can be used to identify agonists for PAQRs of unknown function. Using this system, we found that the proteinaceous hormone adiponectin functions as an agonist of PAQR3, a previously uncharacterized member of this family. This is not surprising given that PAQR3 is most closely related to PAQR1 (AdipoR1) and PAQR2 (AdipoR2), which also sense adiponectin. The identification of adiponectin as an agonist for PAQR3 is of considerable clinical relevance because adiponectin suppresses the proliferation of tumor cells and it has been reported that PAQR3 suppresses tumorigenesis. Thus, the interaction between PAQR3 and adiponectin may help explain the antiproliferative properties of adiponectin.

Keywords::

• Adiponectin receptor
• PAQR3
• RKTG
• yeast
• AdipoR1

Acknowledgments

This work was funded by the University of Florida, Department of Chemistry and the National Institutes of Health (R21DK074812 to TJL).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.