Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system–Epic^® system

Meng WuDepartment of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center (JHICC), Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA

Shunyou LongDepartment of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center (JHICC), Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA

Anthony G. FrutosCorning Incorporated, Science and Technology Division, Corning, New York, USA

Maryna EichelbergerCenter for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, 20892, USA

Min LiDepartment of Neuroscience, High Throughput Biology Center and Johns Hopkins Ion Channel Center (JHICC), Johns Hopkins University, School of Medicine, Baltimore, Maryland, USACorrespondence[email protected]

Ye FangCorning Incorporated, Science and Technology Division, Corning, New York, USA

Abstract

The Epic^® system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.

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Acknowledgments

We thank members of the Li laboratory for valuable comments on this manuscript. This work is supported by a grant from the National Institutes of Health (to M.L. U54 MH084691, RO1 GM078579), and supported in part by Corning Inc.

Declaration of interest: The authors report no conflicts of interest.

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Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system–Epic® system

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Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system–Epic^® system