Structure-based identification of potential novel inhibitors targeting FAM3B (PANDER) causing type 2 diabetes mellitus through virtual screening

Abstract

Type 2 diabetes mellitus is a metabolic disorder that requires potent therapeutic approaches. The FAM3B is a cytokine-like protein also referred to as PANcreatic-DERrived factor (PANDER) which mainly exists in pancreatic islets. In the process of identifying potential inhibitors with the aid of structure-based method PANDER protein is identified as a novel therapeutic target against type 2 diabetes mellitus as it involved in the development of type 2 diabetes by negatively regulating the pancreatic β-cell function and insulin sensitivity in the liver. In the present study, the 3d model of target protein FAM3B was generated by homology modeling technique using the MODELLER9.9 program. The assessment of the structural stability of the 3d model was established by energy minimization technique. The structural quality was evaluated with standard validating protocols. Binding regions of the target protein has been determined by literature and SiteMap tool. In the current study of research, the FAM3B model was subjected to molecular screening with the Asinex-elite database of 14849 output molecules using the Glide virtual screening module in the Schrodinger suite. The final XP descriptor output of 14 molecules was analyzed and prioritized based on molecular interactions at the FAM3B active site. The docking score, binding free energies (Prime MM/GBSA) and bioavailability were undertaken into the consideration to identify lead inhibitors. The identified lead compounds were checked for ADME properties all falling within the permeable ranges. The analysis of results gave the insight to develop the novel therapeutic strategies against type 2 diabetes mellitus.

Keywords:

• FAM3B
• homology modeling
• MM/GBSA
• ADME
• type 2 diabetes mellitus

Acknowledgements

The Authors GL, RB, MD, SN, MB and GS are thankful to Principal and Head, Department of chemistry, University College of Science and Nizam College, Osmania University for providing research facilities to carry out this work. The Author Goverdhan Lanka is thankful to the Council of Scientific andIndustrial Research (CSIR)-INDIA, New Delhi, for the financial support asSenior Research Fellowship (SRF) (File No: 09/132(0841)2015-EMR-I).

Disclosure statement

No potential conflict of interest was reported by the authors.