Cisplatin-loaded albumin nanoparticle and study their internalization effect by using β-cyclodextrin

Abstract

The present study with aim at enhancing the therapeutic and anti-cancer properties of cisplatin (CPT)-loaded bovine serum albumin (BSA) nanoparticles. The BSA nanoparticles containing CPT (CPT-BSANPs) were successfully prepared by the desolvation technique. The physicochemical characterization of the CPT-BSANPs were used by Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The particle size of CPT-BSANPs was found less than 200 nm with 75.02 ± 0.15% entrapment efficiency (EE), while zeta potential and PDI were −17.6 mV and 0.2, respectively. In vitro release behavior of the CPT from the carrier suggests that about 64% of the drug gets released after 48 hrs. The anti-cancer activities of the CPT-BSANPs were tested on MCF-7 cell lines. Our studies show that CPT-BSANPs nanoparticles showed specific targeting and enhanced cytotoxicity to MCF-7 cells when compared to the bare CPT. Thus results suggest that CPT-BSANPs fallowed caveolae-mediated endocytosis, it may become better option for intracellular delivery of anticancer drug.

Keywords:

• Albumin
• nanoparticles: drug delivery
• cisplatin
• MCF-7 cells

Acknowledgments

The authors acknowledge the Translational Research Platform for Veterinary Biologicals (TRPVB), Tamil Nadu Veterinary and Central Instrumental Laboratory (CIL), Dr. H.S. Gour Central University, Sagar (India) for ex vivo characterization supports.

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

Financial support was provided by the Indian Council of Medical Research (ICMR), New Delhi (India) (RT-45/26/2018-Nan/BMS dated 5 April 2018