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Abstract
Cytotoxicity of 14 glove materials representing 4 natural rubber latex, 6 synthetic rubber and 4 synthetic polymeric materials was evaluated using dimethylthiazol diphenyltetrazolium (MTT), agar overlay and filter diffusion tests. Cell responses after contact with extracts of glove materials and contact with glove materials were assessed. One synthetic rubber glove (nitrile rubber) and 2 synthetic polymeric gloves (polyvinyl chloride) were non-toxic in all 3 tests, while 5 synthetic rubbers exhibited varying degrees of cytotoxicity, depending on the test. A severe cytotoxic response to both extracts of natural rubber latex materials and contact with natural rubber latex was verified in the 3 tests, indicating a need for consideration when selecting gloves, or other products, used in close skin contact.