Flask and reactor scale production of plant growth regulators by Inonotus hispidus: optimization, immobilization and kinetic parameters

Abstract

The aims of the presented study are to compare submerged, static, and solid-state fermentation in the production of gibberellic acid (GA₃), indole acetic acid (IAA), and abscisic acid (ABA) by Inonotus hispidus, to optimize with a statistical approach, and to determine the kinetic parameters under flask and reactor conditions. The maximum concentrations of GA₃, (2478.85 ± 68.53 mg/L), ABA, (273.26 ± 6.17 mg/L) and IAA (30.67 ± 0.19 mg/L) were obtained in submerged conditions. After optimization, these values reached 2998.85 ± 28.85, 339.47 ± 5.50, and 34.56 ± 0.25 mg/L, respectively. Immobilization of fungal cells on synthetic fiber, polyurethane foam, and alginate beads resulted in an increase in plant growth regulators (PGR) production by 5.53%− 5.79% under optimized conditions. At the reactor scale, a significant increase was observed for GA₃ concentration, 5441.54 mg/L, which was 2.14 and 1.45 times higher than non-optimized and optimized conditions in the flask scale, respectively. The maximum values for ABA and IAA were 390.39 and 44.79 mg/L, respectively. Although the specific growth rate (µ) decreases relatively from non-optimized flask conditions to optimized reactor conditions, it was observed that the PGR amounts produced per liter medium (r_p) and per gram biomass (Q_p) increased significantly. This is the first report on the synthesis of PGR by Inonotus hispidus which could be crucial for sustainable agriculture.

Keywords:

• Abscisic acid
• gibberellic acid
• indole acetic acid
• Inonotus hispidus
• optimization

Disclosure statement

The authors declare that there are no conflict of interest regarding the publication of this paper.

Additional information

Funding

This work was supported by a grant (112T061) from The Scientific and Technological Research Council of Türkiye (TUBITAK).