11. A Rapid Method for the Purification of the MEFj Strain of Poliomyelitis Virus

ABSTRACT

It was shown that on dialyzing infected brain suspensions against buffers of different pH values the virus comes down in the precipitate at pH 4.3 leaving little virus in the supernatant fluid. The virus in the deposit can now be redispersed in a small volume of phosphate buffer of pH 8.2. The substances responsible for the adsorption of the virus to the deposit can be removed by shaking with chloroform without loss of titre of the virus. The virus in a relatively pure state can now be recovered quantitatively by ultracentrifugation.