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ABSTRACT
By means of precipitin tests, preparations of Type I polio virus have been found to contain at least three antigenically active components. The three precipitin bands have tentatively been correlated to the three distinct components observed in the ultracentrifuge. Type II also contains more than one antigen but the secondary bands are weaker. A lyophilized sample produced a precipitin pattern very similar to that formed by the untreated virus, in spite of the drastic changes in virus infectivity and particle size following this treatment. The Type III virus appeared to be antigenically homogeneous. No cross reactions could be observed between the different virus types and immune sera.