Lipophilicity of Selected Bile Acids, as Determined by TLC. IV. Investigations on CNF₂₅₄ Stationary Phase

Abstract

The aim of our study was to determine the lipophilicity of selected bile acids, i.e., cholic acid (C), glycocholic acid (GC), glycodeoxycholic acid (GDC), chenodeoxycholic (CDC), deoxycholic acid (DC), lithocholic acid (LC), and glycolithocholic acid (GLC) with the use of reversed phase thin‐layer chromatography on CNF₂₅₄ plates (E. Merck, #1.12571) and methanol‐water, organic mixture (acetonitrile–methanol 50∶50, v/v)–water, acetone–water, dioxane–water in different volume compositions as mobile phases. Lipophilicities R_MW and ϕ₀ were compared, both with measured partition coefficients (logP_exp) and the calculated ones (AlogP_S, IAlogP, logP_KOWIN, xlogP, clogP, logP_Rekker). The most significant correlation was found between R_MW and ϕ₀ lipophilic parameters and logP_KOWIN values. Moreover, it was stated that the obtained parameters R_MW and ϕ₀ correlate best with experimental partition coefficients (logP_exp) given after Roda and coauthors. The values of R_MW and ϕ₀ lipophilic parameters obtained on RP18W, RP2, and CNF₂₅₄ plates with the use of the a/m mobile phases, indicate that the investigated bile acids may be listed in order of decreasing lipophilicity as follows: LC>DC≈CDC≈GLC>C≈GDC>GC.

Keywords:

• Bile acids
• CNF₂₅₄ RP‐HPTLC
• Lipophilicity