Determination of Log P_o/w for Catechins and Their Isomers, Oligomers, and Other Organic Compounds by Stationary Phase Controlled High‐Speed Countercurrent Chromatography

Abstract

A new technique, stationary phase volume controlled high‐speed counter‐current chromatography (HSCCC), was used to determine the octanol‐water partition coefficients (P _o/w) of catechins and their isomers, oligomers, and other organic compounds. The stationary phase volume in the CCC column was effectively controlled under hydrodynamic equilibrium system. The log P _o/w values ranging from −1.35 to +3.60 were measured within 21 min using this new HSCCC technology. The linear relationship (correlation coefficient value, r=0.993) was observed between log P _o/w values obtained by the shake‐flask method and those values by the HSCCC method. In this technique, it is possible to inject multiple samples successively into the CCC column at short intervals to measure their retention times without renewing the stationary phase volume.

Keywords:

• High‐speed countercurrent chromatography (HSCCC)
• Stationary phase volume
• Stationary phase fraction (S _F ) controlled HSCCC
• Log P _o/w value
• Catechins

Acknowledgment

The authors are indebted to Dr. Henry M. Fales of National Institutes of Health, Bethesda, Maryland for editing the manuscript with valuable suggestions.