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Abstract
Fluorescence in flow‐injection analysis is described for the simultaneous determination of thiamine, riboflavin, and folic acid. Its detection limit linearity and reproducibility were examined. The kinetic method is based on the enhancing effect of thiamine and riboflavin on the fluorescence generated by oxidizing to thiochrome with alkaline ferricyanide. Different parameters affecting this reaction were thoroughly studied. The procedure was applied to the determination of thiamine and riboflavin and folic acid in human urine samples.
Acknowledgment
Financial support of this work by Chia Nan University of Pharmacy and Science is gratefully acknowledged. CNAC-94-05.