Preparative Isolation and Purification of Prim‐O‐Glucosyl‐Cinmifugin and 4′‐O‐β‐D‐Glucosyl‐5‐O‐Methylvisamminol from Radix saposhnikoviae by High Speed Countercurrent Chromatography

Abstract

A high‐speed counter‐current chromatography (HSCCC) method for separation and purification of prim‐O‐glucosyl‐cinmifugin and 4′‐O‐β‐D‐glucosyl‐5‐O‐methylvisamminol from the extract of Radix saposhnikoviae was developed by using chloroform‐methanol‐water (10∶8∶4, v/v) as the two‐phase solvent system. Prim‐O‐glucosyl‐cinmifugin (21.7 mg) and 44.7 mg of 4′‐O‐β‐D‐glucosyl‐5‐O‐methylvisamminol were produced from 100 mg of the crude sample, both at over 99.0% purity, determined by HPLC. The structures of the isolated compounds were identified by ¹H‐NMR and ¹³C‐NMR.

Keywords:

• Countercurrent chromatography
• Radix saposhnikoviae
• Prim‐O‐glucosyl‐cinmifugin
• 4′‐O‐β‐D‐Glucosyl‐5‐O‐methylvisamminol