Preparative Isolation of Mangiferin from Anemarrhena asphodeloides Rhizomes by Centrifugal Partition Chromatography

Abstract

Mangiferin was separated from rhizomes of Anemarrhena asphodeloides extract using centrifugal partition chromatography (CPC) with a two‐phase solvent system composed of ethyl acetate‐isopropanol‐water (3∶2∶5, v/v). Mangiferin (22.5 mg) was successfully isolated from methanolic extracts (957 mg) in only one step, and the purity of isolated compound was determined to be over 95% by HPLC analysis. The structure of mangiferin was identified by ¹H, ¹³C NMR and ESI‐MS spectral data analysis.

Keywords:

• Centrifugal partition chromatography
• Anemarrhena asphodeloides
• Preparative chromatography
• Mangiferin
• Xanthone glycoside

Acknowledgment

This work was supported by the Korea Research Foundation Grant (KRF‐2003‐015‐E00217).