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Abstract
A sensitive and selective assay of galactose, arabinose, and rhamnose is presented for its determination in mesquite and Arabic gums. Gum samples from mesquite and Arabic were fractionated by hydrophobic affinity chromatography, hydrolyzed, and analyzed by normal bonded phase high performance liquid chromatography with refraction index measurement. The assay demonstrated that it was fast and with good linear and high precision response. The column and detector performance showed good retention, selectivity, and efficiency. Based on replicated analysis of standards over a range of 0–13.33 mg/mL, the method is accurate (95–100%), and precise (CV of 3% daily). The results indicated that both gums consist of three principal fractions. The mesquite tears and native showed higher total carbohydrates content (75.73 and 76.26%) in comparison with Arabic gum (61.81%). The saccharides isolated from the Arabic gum contained galactose, arabinose, and rhamnose. Fraction I of Arabic gum showed a higher rhamnose content (7.24%) in comparison with mesquite (0.89%). Only the hydrolyzed fraction I showed the rhamnose content in mesquite. In the fractions, the content of galactose predominating in the Arabic gum was 25.21–26.35% and arabinose in the mesquite gum was 34.43–53.49%.