TLC Analysis of Corrinoid Compounds in the Halophilic Lactic Acid Bacterium Tetragenococcus halophilus

Abstract

Vitamin B₁₂ content (3.1±0.8 µg/100 g wet weight) of the lactic acid bacterium Tetragenococcus halophilus IAC‐ks6, isolated from a fermented skipjack viscera, was determined using the microbiological vitamin B₁₂ assay method with Lactobacillus delbrueckii subsp. lactis ATCC 7830. On the basis of silica gel 60 TLC and C₁₈ reversed‐phase HPLC, true vitamin B₁₂ predominated in the bacterial cells and functioned as each cofactor of the vitamin B₁₂‐dependent enzymes, methionine synthase and ribonucleotide reductase.

Keywords:

• HPLC
• Salted and fermented skipjack viscera
• Tetragenococcus halophilus
• TLC
• Vitamin B₁₂