Characterization of TiO₂/SiO₂ Based Stationary Phase for RP‐HPLC

Abstract

In this paper, we prepared a reversed‐phase support by bonding octadecyltrichlorosilane onto TiO₂/SiO₂ particles, which were prepared by a layer‐by‐layer self‐assembly technique and consists of micrometer‐sized silica spheres as core and nanometer‐sized titania particles as surface coating. The chromatographic behavior of the support was studied in detail. The support acted as a true reversed chromatographic stationary phase and had a hydrophobic selectivity. Further more, it had a comparable high carbon content of 11.51% and showed good stability in the basic solution of pH 10.

Keywords:

• Novel RP‐HPLC packing
• Octadecyl‐bonded
• TiO₂/SiO₂
• Nanoparticle
• Layer‐by‐layer self‐assemble

Acknowledgments

The authors acknowledge financial support to “Hundreds of Talents Program” to Y.M.L. by the Chinese Academy of Sciences and also acknowledge financial support of the National Nature Science Foundation of China (Grant:20375045).