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Abstract
The quantitation of galanin from rat plasma was conducted using protein precipitation and LC/MS. This method was externally calibrated, as no suitable internal standard was available. Galanin was detected in the positive ion mode by selected ion monitoring of the 3+‐charge state. The lower limit of quantitation was 10 ng/mL and calibration curves were linear over the concentration range from 10 to 1,000 ng/mL. Within and between run precision and accuracy was less than 12% at all validation points. The method was demonstrated by a limited pharmacokinetic study of galanin in rats.