Study of SPE Conditions for CE Determination of Tricyclic Antidepressants in Body Fluids

Abstract

The optimal solid‐phase extraction conditions for six tricyclic antidepressants present in body fluids have been studied. The compounds were separated and detected by capillary electrophoresis with UV spectrophotometry. In the examinations, three sorbents, five pretreatments of body fluids, and four extraction procedures were taken into account. On the basis of preliminary experiments, the best starting conditions were chosen for further optimization of such factors as sorbent mass and volumes of conditioning, washing, and eluent reagents. In the optimized conditions, the average extraction recoveries and their repeatability for all drugs present in whole blood and serum have been evaluated.

Keywords:

• Tricyclic antidepressants
• Solid‐phase extraction
• Capillary electrophoresis
• Whole blood
• Serum
• Urine