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Abstract
A simple high performance liquid chromatographic (HPLC) method was developed for the analysis of ethamsylate (ET) and mefenamic acid (MA) in bulk drug and tablets. This method showed good resolution for both the drugs. An isocratic reverse‐phase HPLC assay was used with PDA detection. Some solvent systems prepared using methanol or acetonitrile and water or buffer systems with different pH values were tested. Best resolution has been determined using a Luna C8 column with acetonitrile and water (60∶40,V/V) as mobile phase adjusted to pH 2.5 with acetic acid. Ibuprofen was used as an internal standard, as use of internal standard makes the analyses more accurate. Samples were eluted isocratically with mobile phase at flow rate 1.0 mL min−1 and detected at 300 nm.