Separation of Tribenzylhydrogermanium Nitrile Derivatives by Means of HPLC with Participation of Π‐Π Interactions

Abstract

This paper is a continuation of an investigation concerning optimization of chromatography of tribenzylhydrogermanium nitrile derivatives. Organic derivatives of germanium compounds cause numerous difficulties during determinations, because of easy precipitation on chromatographic columns. Optimal conditions of separation and determination of 3‐tribenzylgermanylbutyronitrile and 4‐tribenzylgermanylbutyronitrile have been elaborated, taking advantage of π‐π interactions between the stationary phase and analyzed compounds. In the investigation three aryl stationary phases and various compositions and contents of the mobile phase were considered. Obtained results showed, that the best selectivity yielded a column containing porous graphitized coal. The highest separation factor (2.85) has been obtained using such a column and dichloromethane, as a mobile phase and flow 0.3 mL · min⁻¹. A significantly lower separation factor (1.30) for the same mobile phase gave the naphthylpropyl stationary phase. Obtained results confirm a predominating influence of π‐π interactions.

Keywords:

• Aryl stationary phases
• Isomers
• Tribenzylgermanes
• Chromatography