Separation and Determination of Major Bioactive Components in Radix Tinosporae by Gradient Pressurized Capillary Electrochromatography

Abstract

An approach of gradient pressurized capillary electrochromatography with UV detection was utilized for the separation and determination of major bioactive components in Radix Tinosporae extract, including two botanical steroids and three alkaloids. The assay was performed on a 30 cm ODS capillary column with acetonitrile‐water gradient elution. Under the optimum conditions, five investigated compounds were eluted within 25 minutes, and were achieved with acceptable linearity, precision, repeatability, and accuracy. The method was successfully applied to analyze five major components in various Radix Tinosporae samples, and was sufficed to be utilized as a novel quality control approach for traditional Chinese medicine.

Keywords:

• Pressurized capillary electrochromatography
• Alkaloid
• Radix Tinosporae
• Traditional Chinese medicine

Acknowledgments

This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) and partly supported by Scientific Foundation of Shanghai, China (NO. 04DZ19856, 04DZ19857, 04DZ19842, and 05DZ19733).