Separation of Ginsenosides at Elevated Temperature by Ultra High Pressure Liquid Chromatography

Abstract

Separation of seven ginsenosides at elevated temperature by ultra high pressure liquid chromatography (UHPLC) on reversed phase C_18‐ bonded silica stationary phase was studied. All separations were carried out with a binary mobile phase consisting of CH₃CN/H₂O in isocratic elution mode. It was found that increasing the column temperature resulted in efficient separation and complete resolution of seven ginsenosides. Generally, the separation efficiency increased as a function of temperature. The effect of the mobile phase velocity on the separation efficiency was also investigated.

Keywords:

• Ultra High Pressure Liquid Chromatography
• Ginsenosides
• Elevated temperature
• van't Hoff plot
• Isocratic elution

Acknowledgment

The authors thank Prof. A. Y. El‐Naggar, Egyptian Petroleum Research Institute, Nasr City, Egypt for his advice and criticism.

Notes

^†On leave from the Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan.

^‡On leave from Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Philippines.