57
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

Identification of Drugs by Ultra Fast Liquid Chromatography/Electrospray Ionization‐Quadrupole Ion Trap/Time‐of‐Flight Mass Spectrometry

, , , &
Pages 23-37 | Received 26 Feb 2007, Accepted 09 Apr 2007, Published online: 06 Nov 2007
 

Abstract

Hybrid quadrupole ion trap/time‐of‐flight mass spectrometry (QIT/TOFMS) was applied for detection of ultra fast liquid chromatography (UFLC). The system performance for fast qualitative analysis was evaluated using test drugs. The test drugs were separated on a C18 column (30 mm×2.0 mm I.D., particle size: 2.2 µm) with fast linear gradient elution using 0.1% formic acid and acetonitrile as mobile phase. The flow rate was set at 0.5 mL/min and the analysis cycle time was 4.5 min. Relative standard deviations (RSDs) of retention time and peak area for each drug (200 pg each) were 0.2% or better, lower than 3%, respectively. Mass accuracy of each compound was found to be 3.2 ppm or better. Rapid positive to negative polarity switching mode was demonstrated, showing good mass accuracy below 5 ppm. Moreover, an MS3 measurement was carried out and the formulae of compounds were confirmed using formula prediction software.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.