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Abstract
Presented in this paper are results of investigations concerning optimization of conditions of separation and determination of two newly obtained hyper coordinated silicates, which belong to the group of ES‐silanates. To this process were subjected: 1‐[N‐(phenylmethyl)aminiomethyl]spirobi(sila‐2,5‐dioxa‐4,4‐dimethylcyclopentane‐3‐on)ate and 1‐[N‐(phenylmethyl)aminiomethyl]spirobi(sila‐2,5‐dioxacyclopentane‐3‐on)ate. The analyzed compounds show biological activity. Three stationary phases (octadecyl, octyl and naphthylpropyl), two mobile phases (acetonitrile, dichloromethane) with various flow were tested. The highest selectivity and separation factors (α=1.27) were obtained using the naphthylpropyl column and the mobile phase consisting of pure acetonitrile. The octadecyl (which is recommended as standard) phase did not enable satisfactory results.