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Abstract
A novel spiral tube support is introduced which allows accommodating a multlilayer spiral column made of a long piece of fluorinated plastic tubing. Two spiral columns tested in this study consist of 1.6 mm ID and 0.85 mm ID FEP (fluorinated ethylenepropylene) and PTFE (polytetrafluoroethylene) tubing with the total capacity of 100 mL and 40 mL, respectively. Performance of these spiral columns was examined with a two phase solvent system composed of 1‐butanol/acetic acid/water at a volume ratio of 4∶1∶5 using tryptophyl‐tyrosine (try‐tyr) and valyl‐tyrosine (val‐tyr) as test samples under various revolution speeds and flow rates. Among 4 different elution modes tested, L‐I‐T (lower phase pumped from the internal head end of the spiral column) and U‐O‐H (upper phase pumped from the external tail of the spiral column) at a low flow rate produced the best results especially for both columns. The highest peak resolution (Rs) of over 3.5 was obtained from U‐O‐H at a flow rate of 1 mL/min with 74% stationary phase retention. At a flow rate of 5 mL/min, the highest revolution speed at 1,200 rpm improved the peak resolution in both elution modes. The present system may be useful for purification of various polar compounds in biomedical researches.