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Abstract
A normal phase high performance liquid chromatographic method was established for the simultaneous determination of tanshinones in Salvia miltiorrhiza Bunge. Tanshinones, including cryptotanshinone, tanshinone I, and tanshinone IIA were successfully separated on a silica column using the mixture of n-hexane and dichloromethane (96/4, v/v) as mobile phase. The results of the quantitative experiments proved that methanol is the best solvent in the five common solvents. Linear equations revealed good linear relationship between the peak areas of tanshinones and their concentrations. The effect of the dipping time and ultrasonic time were measured, the trend lines dependent on the linear equations were drawn, and the ultrasonic condition was better than the dipping condition.
Notes
X denotes concentration (µg/mL) of the tanshinones, Y denotes peak area.
The liquid/solid ratio is 50:1, “∗”not detected.
