Analytical Evaluation of Visualizing Reagents used to Detect Ibuprofen on Thin Layers

Abstract

Six dyes as new visualizing reagents, namely, gentian violet, methylene violet, methylene blue, methyl green, malachite green, and Janus blue, have been used to detect ibuprofen on silica gel 60F₂₅₄. Rhodamine B was used as the comparative visualizing reagent. The limit of detection (detectability), detection index, broadening index, modified contrast index, densitometric visualizing index, and linearity range were determined for ibuprofen following use of these visualizing reagents. It was stated that the earlier proposed densitometric visualizing index is an objective parameter describing the applied visualizing reagents. The best detection method for ibuprofen is the densitometric method without using a visualizing reagent. Among all studied new visualizing reagents, methylene violet is the best for detecting ibuprofen. The obtained visualizing effects and non-destructive properties of the applied visualizing reagents, in relation to investigated ibuprofen, indicate that progress in the range of analysis of ibuprofen on thin layer has taken place. This fact has definite analytical, pharmaceutical, and physicochemical significance.

Keywords:

• Broadening index
• Densitometric visualizing index
• Densitometry
• Detectability
• Detection index
• Dyes
• Ibuprofen
• Modified contrast index
• New visualizing reagents
• NP-TLC
• Pharmaceutical analysis

ACKNOWLEDGMENT

This research was financed by the Ministry of Science and Higher Education by resources reserved for science in the years 2005–2008 as research project No. 3T09A 155 29.

Notes

^a Intensity of all absorption maximum is equal to 95 AU.